Immobilized enzyme microreactors for analysis of tryptic peptides in β-casein and β-lactoglobulin.

ABSTRACT: In this study, our primary objective was to develop an effective analytical method for studying trypsin-digested peptides of two proteins commonly found in cow's milk: β-casein (βCN) and β-lactoglobulin (βLG). To achieve this, we employed two distinct approaches: traditional in-gel protein digestion and protein digestion using immobilized enzyme microreactors (μ-IMER). Both methods utilized ZipTip pipette tips filled with C18 reverse phase media for sample concentration. The μ-IMER was fabricated through a multi-step process that included preconditioning the capillary, modifying its surface, synthesizing a monolithic support, and further surface modification. Its performance was evaluated under HPLC chromatography conditions using a small-molecule trypsin substrate (BAEE). Hydrolysates fro

SUBMITTER: Rodzik A 

PROVIDER: S-EPMC10545664 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature