A replicative recombinant HPV16 E7 expression virus upregulates CD36 in C33A cells.

ABSTRACT:

Objective

In past decades, the role of high-risk HPV (HR-HPV) infection in cancer pathogenesis has been extensively studied. The viral E7 protein expressed in pre-malignant cells has been identified as an ideal target for immunological intervention. However, the cultivation of HPV in vitro remains a significant challenge, as well as the lack of methods for expressing the HPV E7 protein and generating replication-competent recombinant viral particles, which posed a major obstacle to further exploration of the function and carcinogenic mechanisms of the E7 oncoprotein. Therefore, it is imperative to investigate novel methodologies to construct replication-competent recombinant viral particles that express the HPV E7 protein to facilitate the study of its function.

Methods</

SUBMITTER: Shao Y 

PROVIDER: S-EPMC10545859 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature