Unravelling the role of immune cells and FN1 in the recurrence and therapeutic process of skull base chordoma.

ABSTRACT:

Background

Skull base chordoma is a rare and aggressive tumour of the bone that has a high likelihood of recurrence. The fundamental differences in single cells between primary and recurrent lesions remain poorly understood, impeding development of effective treatment approaches.

Methods

To obtain an understanding of the differences in single cells between primary and recurrent chordomas, we performed single-cell RNA sequencing and T-cell/B-cell receptor (BCR) sequencing. This allowed us to delineate the differences between the two types of tumour cells, tumour-infiltrating lymphocytes, myeloid cells, fibroblasts and B cells. Copy number variants (CNVs) were detected and compared between the tumour types to assess heterogeneity. Selected samples were subjected to immunohisto

SUBMITTER: Huo X 

PROVIDER: S-EPMC10545891 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature