Single-cell assignment using multiple-adversarial domain adaptation network with large-scale references.

ABSTRACT: The rapid accumulation of single-cell RNA-seq data has provided rich resources to characterize various human cell populations. However, achieving accurate cell-type annotation using public references presents challenges due to inconsistent annotations, batch effects, and rare cell types. Here, we introduce SELINA (single-cell identity navigator), an integrative and automatic cell-type annotation framework based on a pre-curated reference atlas spanning various tissues. SELINA employs a multiple-adversarial domain adaptation network to remove batch effects within the reference dataset. Additionally, it enhances the annotation of less frequent cell types by synthetic minority oversampling and fits query data with the reference data using an autoencoder. SELINA culminates in the creation of a

SUBMITTER: Ren P 

PROVIDER: S-EPMC10545911 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature