Obesity accelerates endothelial-to-mesenchymal transition in adipose tissues of mice and humans.

ABSTRACT:

Introduction

Vascular dysfunction and chronic inflammation are characteristics of obesity-induced adipose tissue dysfunction. Proinflammatory cytokines can drive an endothelial-to-mesenchymal transition (EndoMT), where endothelial cells undergo a phenotypic switch to mesenchymal-like cells that are pro-inflammatory and pro-fibrotic. In this study, we sought to determine whether obesity can promote EndoMT in adipose tissue.

Methods

Mice in which endothelial cells are lineage-traced with eYFP were fed a high-fat/high-sucrose (HF/HS) or Control diet for 13, 26, and 52 weeks, and EndoMT was assessed in adipose tissue depots as percentage of CD45^-CD31^-Acta2⁺ mesenchymal-like cells that were eYFP ⁺. EndoMT was also assessed in human adip

SUBMITTER: Chavkin NW 

PROVIDER: S-EPMC10546194 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature