Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.

ABSTRACT:

Significance

The dual-wedge prism (DWP)-based spectroscopic single-molecule localization microscopy (sSMLM) system offers improved localization precision and adjustable spectral or localization performance, but its nonlinear spectral dispersion presents a challenge. A systematic method can help understand the challenges and thereafter optimize the DWP system's performance by customizing the system parameters to maximize the spectral or localization performance for various molecular labels.

Aim

We developed a Monte Carlo (MC)-based model that predicts the imaging output of the DWP-based sSMLM system given different system parameters.

Approach

We assessed our MC model's localization and spectral precisions by comparing our simulation against theoretical equations and fl

SUBMITTER: Yeo WH 

PROVIDER: S-EPMC10546470 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature