Potent antitumor efficacy of human dental pulp stem cells armed with YSCH-01 oncolytic adenovirus.

ABSTRACT:

Background

Systemic administration of oncolytic adenovirus for cancer therapy is still a challenge. Mesenchymal stem cells as cell carriers have gained increasing attention in drug delivery due to their excellent tumor tropism, immunosuppressive modulatory effects, and paracrine effects. However, the potential of human dental pulp stem cells (hDPSCs) loaded with oncolytic adenovirus for cancer biotherapy has not been investigated yet.

Methods

The stemness of hDPSCs was characterized by FACS analysis and Alizarin red staining, Oil Red O staining, and immunofluorescence assays. The biological fitness of hDPSCs loaded with oncolytic adenovirus YSCH-01 was confirmed by virus infection with different dosages and cell viability CCK-8 assays. Additionally, the expression of CAR rec

SUBMITTER: He X 

PROVIDER: S-EPMC10546667 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature