Dataset Information

Mechanism of Shugan Yidan fan, a Chinese herbal formula, in rat model of premature ejaculation.

ABSTRACT:

Background

Premature ejaculation (PE) is one of the most common forms of sexual dysfunction in men, and multimodal therapeutic regimens should be considered to treat the condition. We developed a Chinese medicine herbal medicine, Shugan Yidan fang that had a significant clinical effect on PE patients, extending the time between penetration and ejaculation. However, the mechanism of this formula remains unclear. There is evidence that PE is associated with peripheral neuropathology, and the actions of dopamine (DA) and 5-hydroxytryptamine (5-HT). The aim of this study was to investigate the mechanism of Shugan Yidan fang's effect on PE through the relationship between sexual behavioristics and the level of neurotransmitters and dopamine receptors (DARs).

Results

We showed that the male PE groups had a significant PE phenotype compared to healthy rats. Treatment with Shugan Yidan fang improved the behavioristics of the PE rats, and reduced the expression of DAR mRNA and protein while improving dopamine transporter levels.

Conclusions

Our study provided evidence for the beneficial effect of Shugan Yidan fang in PE therapy, and proposed a preliminary potential mechanism for the clinical application of the formula.

SUBMITTER: Han Q

PROVIDER: S-EPMC10546682 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Publications

Mechanism of Shugan Yidan fan, a Chinese herbal formula, in rat model of premature ejaculation.

Han Qiang Q Guo Jun J Wang Renyuan R Li Jiangminzi J Wang Fu F Gao Qinghe Q Zhang Jiwei J Wang Hetian H Zeng Yin Y

Basic and clinical andrology 20231003 1

<h4>Background</h4>Premature ejaculation (PE) is one of the most common forms of sexual dysfunction in men, and multimodal therapeutic regimens should be considered to treat the condition. We developed a Chinese medicine herbal medicine, Shugan Yidan fang that had a significant clinical effect on PE patients, extending the time between penetration and ejaculation. However, the mechanism of this formula remains unclear. There is evidence that PE is associated with peripheral neuropathology, and t ...[more]

PMID: 37784033

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Project description:Backgroundpremature ovarian failure (POF) is a significant condition characterized by the early loss of ovarian function, often leading to infertility and other health complications. Traditional Chinese Medicine (TCM) formulations, such as Chaihu Shugan San and Zuogui Yin, have been used to address various gynecological disorders. This study aims to evaluate the therapeutic effects of Chaihu Shugan San, Zuogui Yin, and their combination on POI in a rat model and to elucidate the underlying mechanisms involving the PI3K/Akt/mTOR signaling pathway.MethodsIn this study, a premature ovarian failure (POF) rat model was established using chronic unpredictable mild stress (CUMS) combined with cyclophosphamide injections. Rats were treated with Chaihu Shugan San, Zuogui Yin, or their combination. Key outcomes, including ovarian granulosa cell proliferation, follicular integrity, and hormonal levels (FSH and E2), were assessed. Histopathological changes in ovarian tissue were evaluated using hematoxylin and eosin (H&E) staining, while apoptosis was detected through immunohistochemistry and Western blot analysis of proteins such as Bcl-2, Bax, Caspase3, and Caspase9. The activity of the PI3K/Akt/mTOR signaling pathway was quantified using Western blot, and statistical significance was determined (P < 0.05).ResultsThe results showed that all three treatments significantly improved ovarian function in the POF rat model. Compared to the model group, Chaihu Shugan San, Zuogui Yin, and their combination increased granulosa cell proliferation (P < 0.01), reduced follicular atresia (P < 0.05), and normalized hormone levels (FSH: reduced by 36.5 %; E2: increased by 42.8 %, P < 0.05). Histopathological analysis confirmed preserved follicular structure and reduced apoptosis in treated groups. Western blot results revealed enhanced PI3K/Akt/mTOR pathway activity, with upregulated Bcl-2 expression (P < 0.01), downregulated Bax expression (P < 0.01), and decreased Caspase3 and Caspase9 levels (P < 0.05). Additionally, CyclinD2 and P70S6 K expression were significantly increased in treated groups, suggesting enhanced cell cycle progression and protein synthesis.ConclusionChaihu Shugan San, Zuogui Yin, and their combination exhibit potential efficacy as treatments for POF in rats by enhancing granulosa cell proliferation, preserving follicular integrity, and inhibiting apoptosis. These therapeutic effects are mediated through the activation of the PI3K/Akt/mTOR signaling pathway and regulation of apoptosis-related proteins. This study highlights the importance of further exploring apoptosis-related pathways to understand the therapeutic mechanisms of these TCM formulations, providing a foundation for new clinical strategies to manage POF and related reproductive health issues.

Dataset Information

Mechanism of Shugan Yidan fan, a Chinese herbal formula, in rat model of premature ejaculation.

Background

Results

Conclusions

Publications

Mechanism of Shugan Yidan fan, a Chinese herbal formula, in rat model of premature ejaculation.

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