Automating high-throughput screening for anthracnose resistance in common bean using allele specific PCR.

ABSTRACT:

Background

Common beans (Phaseolus vulgaris L.) provide important protein and calories globally. Anthracnose (Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara, 1889) is a major disease in common bean and causes significant yield losses in bean production areas. Screening for markers linked to known disease resistance genes provides useful information for plant breeders to develop improved common bean varieties. The Kompetitive Allele Specific PCR (KASP) assay is an affordable genetic screening technique that can be used to accelerate breeding programs, but manual DNA extraction and KASP assay preparation are time-consuming. Several KASP markers have been developed for genes involved in resistance to bean anthracnose, which can reduce yield by up to 100%, but their use

SUBMITTER: Zaleski-Cox M 

PROVIDER: S-EPMC10546687 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature