Analysis of resistance genes of carbapenem-resistant Providencia rettgeri using whole genome sequencing.

ABSTRACT:

Objective

This study aimed to investigate the clinical infection characteristics and analyze the resistance gene carrying status of carbapenem-resistant Providencia rettgeri via whole genome sequencing (WGS).

Methods

Carbapenem-resistant P. rettgeri were collected from clinical patients between January 2020 and December 2021, and their susceptibility to 19 antimicrobial drugs was determined using the VITEK 2 Compact system and Kirby-Bauer (KB) disk diffusion method. The Illumina platform was used to perform WGS of the P. rettgeri isolates, and the resistance genes carried by the Carbapenem-resistant P. rettgeri strains were detected via ABRicate software. The phylogenetic tree was constructed by thirty-four strains including twenty-eight strains downloaded from NCBI database

SUBMITTER: Liu M 

PROVIDER: S-EPMC10546784 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature