Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell-neuron cocultures.

ABSTRACT: Myelinating Schwann cell (SC)-dorsal root ganglion (DRG) neuron cocultures are an important technique for understanding cell-cell signalling and interactions during peripheral nervous system (PNS) myelination, injury, and regeneration. Although methods using rat SCs and neurons or mouse DRG explants are commonplace, there are no established protocols for compartmentalised myelinating cocultures with dissociated mouse cells. There consequently is a need for a coculture protocol that allows separate genetic manipulation of mouse SCs or neurons, or use of cells from different transgenic animals to complement in vivo mouse experiments. However, inducing myelination of dissociated mouse SCs in culture is challenging. Here, we describe a new method to coculture dissociated mouse SCs and DRG neur

SUBMITTER: Mutschler C 

PROVIDER: S-EPMC10546878 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature