Autoantibodies identify primary Sjogren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B.

ABSTRACT:

Objective

Identify autoantibodies in anti-Ro/SS-A negative primary Sjögren's syndrome (SS).

Methods

This is a proof-of-concept, case-control study of SS, healthy (HC) and other disease (OD) controls. A discovery dataset of plasma samples (n=30 SS, n=15 HC) was tested on human proteome arrays containing 19 500 proteins. A validation dataset of plasma and stimulated parotid saliva from additional SS cases (n=46 anti-Ro⁺, n=50 anti-Ro^-), HC (n=42) and OD (n=54) was tested on custom arrays containing 74 proteins. For each protein, the mean+3 SD of the HC value defined the positivity threshold. Differences from HC were determined by Fisher's exact test and random forest machine learning using 2/3 of the validation dataset for training and 1/3 for testing. Ap

SUBMITTER: Longobardi S 

PROVIDER: S-EPMC10546962 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature