Allele-specific RNA N ⁶-methyladenosine modifications reveal functional genetic variants in human tissues.

ABSTRACT: An intricate network of cis- and trans-elements acts on RNA N ⁶-methyladenosine (m⁶A), which in turn may affect gene expression and, ultimately, human health. A complete understanding of this network requires new approaches to accurately measure the subtle m⁶A differences arising from genetic variants, many of which have been associated with common diseases. To address this gap, we developed a method to accurately and sensitively detect transcriptome-wide allele-specific m⁶A (ASm⁶A) from MeRIP-seq data and applied it to uncover 12,056 high-confidence ASm⁶A modifications from 25 human tissues. We also identified 1184 putative functional variants for ASm⁶A regulation, a subset of which we experi

SUBMITTER: Cao S 

PROVIDER: S-EPMC10547253 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature