Chromatin loop dynamics during cellular differentiation are associated with changes to both anchor and internal regulatory features.

ABSTRACT: Three-dimensional (3D) chromatin structure has been shown to play a role in regulating gene transcription during biological transitions. Although our understanding of loop formation and maintenance is rapidly improving, much less is known about the mechanisms driving changes in looping and the impact of differential looping on gene transcription. One limitation has been a lack of well-powered differential looping data sets. To address this, we conducted a deeply sequenced Hi-C time course of megakaryocyte development comprising four biological replicates and 6 billion reads per time point. Statistical analysis revealed 1503 differential loops. Gained loop anchors were enriched for AP-1 occupancy and were characterized by large increases in histone H3K27ac (over 11-fold) but relatively smal

SUBMITTER: Bond ML 

PROVIDER: S-EPMC10547260 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature