Mitogen-induced defective mitosis transforms neural progenitor cells.

ABSTRACT:

Background

Chromosome instability (CIN) with recurrent copy number alterations is a feature of many solid tumors, including glioblastoma (GBM), yet the genes that regulate cell division are rarely mutated in cancers. Here, we show that the brain-abundant mitogen, platelet-derived growth factor-A (PDGFA) fails to induce the expression of kinetochore and spindle assembly checkpoint genes leading to defective mitosis in neural progenitor cells (NPCs).

Methods

Using a recently reported in vitro model of the initiation of high-grade gliomas from murine NPCs, we investigated the immediate effects of PDGFA exposure on the nuclear and mitotic phenotypes and patterns of gene and protein expression in NPCs, a putative GBM cell of origin.

Results

NPCs divided abnormally in defin

SUBMITTER: Omairi HK 

PROVIDER: S-EPMC10547526 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature