Intra- and interchromosomal contact mapping reveals the Igh locus has extensive conformational heterogeneity and interacts with B-lineage genes.

ABSTRACT: To produce a diverse antibody repertoire, immunoglobulin heavy-chain (Igh) loci undergo large-scale alterations in structure to facilitate juxtaposition and recombination of spatially separated variable (V_H), diversity (D_H), and joining (J_H) genes. These chromosomal alterations are poorly understood. Uncovering their patterns shows how chromosome dynamics underpins antibody diversity. Using tiled Capture Hi-C, we produce a comprehensive map of chromatin interactions throughout the 2.8-Mb Igh locus in progenitor B cells. We find that the Igh locus folds into semi-rigid subdomains and undergoes flexible looping of the V_H genes to its 3' end, reconciling two views of locus organization. Deconvolution of single Igh locus conformations using polymer s

SUBMITTER: Mielczarek O 

PROVIDER: S-EPMC10548092 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature