Cascaded domain multiphoton spatial frequency modulation imaging.

ABSTRACT:

Significance

Multiphoton microscopy is a powerful imaging tool for biomedical applications. A variety of techniques and respective benefits exist for multiphoton microscopy, but an enhanced resolution is especially desired. Additionally multiphoton microscopy requires ultrafast pulses for excitation, so optimization of the pulse duration at the sample is critical for strong signals.

Aim

We aim to perform enhanced resolution imaging that is robust to scattering using a structured illumination technique while also providing a rapid and easily repeatable means to optimize group delay dispersion (GDD) compensation through to the sample.

Approach

Spatial frequency modulation imaging (SPIFI) is used in two domains: the spatial domain (SD) and the wavelength domain (WD). The

SUBMITTER: Scarbrough D 

PROVIDER: S-EPMC10548116 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature