Development and evaluation of nanobody tracers for noninvasive nuclear imaging of the immune-checkpoint TIGIT.

ABSTRACT:

Introduction

T cell Ig and ITIM domain receptor (TIGIT) is a next-generation immune checkpoint predominantly expressed on activated T cells and NK cells, exhibiting an unfavorable prognostic association with various malignancies. Despite the emergence of multiple TIGIT-blocking agents entering clinical trials, only a fraction of patients responded positively to anti-TIGIT therapy. Consequently, an urgent demand arises for noninvasive techniques to quantify and monitor TIGIT expression, facilitating patient stratification and enhancing therapeutic outcomes. Small antigen binding moieties such as nanobodies, are promising candidates for such tracer development.

Methods

We generated a panel of anti-human or anti-mouse TIGIT nanobodies from immunized llamas. In addition, we desi

SUBMITTER: Zeven K 

PROVIDER: S-EPMC10548220 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature