Growth-coupled anaerobic production of isobutanol from glucose in minimal medium with Escherichia coli.

ABSTRACT:

Background

The microbial production of isobutanol holds promise to become a sustainable alternative to fossil-based synthesis routes for this important chemical. Escherichia coli has been considered as one production host, however, due to redox imbalance, growth-coupled anaerobic production of isobutanol from glucose in E. coli is only possible if complex media additives or small amounts of oxygen are provided. These strategies have a negative impact on product yield, productivity, reproducibility, and production costs.

Results

In this study, we propose a strategy based on acetate as co-substrate for resolving the redox imbalance. We constructed the E. coli background strain SB001 (ΔldhA ΔfrdA ΔpflB) with blocked pathways from glucose to alternative fermentation products but

SUBMITTER: Boecker S 

PROVIDER: S-EPMC10548627 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature