Isoform-specific optical activation of kinase function reveals p38-ERK signaling crosstalk.

ABSTRACT: Evolution has diversified the mammalian proteome by the generation of protein isoforms that originate from identical genes, e.g., through alternative gene splicing or post-translational modifications, or very similar genes found in gene families. Protein isoforms can have either overlapping or unique functions and traditional chemical, biochemical, and genetic techniques are often limited in their ability to differentiate between isoforms due to their high similarity. This is particularly true in the context of highly dynamic cell signaling cascades, which often require acute spatiotemporal perturbation to assess mechanistic details. To that end, we describe a method for the selective perturbation of the individual protein isoforms of the mitogen-activated protein kinase (MAPK) p38.

SUBMITTER: Zhou W 

PROVIDER: S-EPMC10549237 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature