RiPP enzyme heterocomplex structure-guided discovery of a bacterial borosin α-N-methylated peptide natural product.

ABSTRACT: Amide peptide backbone methylation is a characteristic post-translational modification found in a family of ribosomally synthesized and post-translationally modified peptide natural products (RiPPs) called borosins. Previously, we bioinformatically identified >1500 putative borosin pathways in bacteria; however, none of the pathways were associated with a known secondary metabolite. Through in-depth characterization of a borosin pathway in Shewanella oneidensis MR-1, we have now identified a bacterially derived borosin natural product named Shewanellamide A. Borosin identification was facilitated by the creation and analysis of a series of precursor variants and crystallographic interrogation of variant precursor and methyltransferase complexes. Along with assaying two proteases fro

SUBMITTER: Crone KK 

PROVIDER: S-EPMC10549244 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature