High-Throughput Method for Wide-Coverage and Quantitative Phenolic Fingerprinting in Plant-Origin Foods and Urine Samples.

ABSTRACT: The use of mass spectrometry is currently widespread in polyphenol research because of its sensitivity and selectivity, but its usual high cost, reduced robustness, and nonavailability in many analytical laboratories considerably hinder its routine implementation. Herein, we describe the optimization and validation of a high-throughput, wide-coverage, and robust metabolomics method based on reversed-phase ultra-high-performance liquid chromatography with diode array detection for the identification and quantification of 69 phenolic compounds and related metabolites covering a broad chemical space of the characteristic secondary metabolome of plant foods. The method was satisfactorily validated following the Food and Drug Administration guidelines in terms of linearity (4-5 orders of magnit

SUBMITTER: Gonzalez-Dominguez R 

PROVIDER: S-EPMC10550202 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature