Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells.

ABSTRACT: Cell spreading and migration play central roles in many physiological and pathophysiological processes. We have previously shown that MFN2 regulates the migration of human neutrophil-like cells via suppressing Rac activation. Here, we show that in mouse embryonic fibroblasts, MFN2 suppresses RhoA activation and supports cell polarization. After initial spreading, the wild-type cells polarize and migrate, whereas the Mfn2^-/- cells maintain a circular shape. Increased cytosolic Ca²⁺ resulting from the loss of Mfn2 is directly responsible for this phenotype, which can be rescued by expressing an artificial tether to bring mitochondria and endoplasmic reticulum to close vicinity. Elevated cytosolic Ca²⁺ activates Ca²⁺/calmodulin-dependent prot

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10550287 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature