Archival skin biopsy specimens as a tool for miRNA-based diagnosis: Technical and post-analytical considerations.

ABSTRACT: Archived specimens, taken by standardized procedures in clinical practice, represent a valuable resource in translational medicine. Their use in retrospective molecular-based studies could provide disease and therapy predictors. Microfluidic array is a user-friendly and cost-effective method allowing profiling of hundreds of microRNAs (miRNAs) from a low amount of RNA. However, even though tissue miRNAs may include potentially robust biomarkers, non-uniformed post-analytical pipelines could hinder translation into clinics. In this study, epidermal RNA from archival skin biopsy specimens was isolated from patients with peripheral neuropathy and healthy individuals. Unbiased miRNA profiling was performed using RT-qPCR-based microfluidic array. We demonstrated that RNA obtained from archival

SUBMITTER: Andelic M 

PROVIDER: S-EPMC10550798 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature