Peristalsis-Associated Mechanotransduction Drives Malignant Progression of Colorectal Cancer.

ABSTRACT:

Introduction

In the colorectal cancer (CRC) tumor microenvironment, cancerous and precancerous cells continuously experience mechanical forces associated with peristalsis. Given that mechanical forces like shear stress and strain can positively impact cancer progression, we explored the hypothesis that peristalsis may also contribute to malignant progression in CRC. We defined malignant progression as enrichment of cancer stem cells and the acquisition of invasive behaviors, both vital to CRC progression.

Methods

We leveraged our peristalsis bioreactor to expose CRC cell lines (HCT116), patient-derived xenograft (PDX1,2) lines, or non-cancerous intestinal cells (HIEC-6) to forces associated with peristalsis in vitro. Cells were maintained in static control conditions or expo

SUBMITTER: Clevenger AJ 

PROVIDER: S-EPMC10550901 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature