Structural mechanisms of the human cardiac sodium-calcium exchanger NCX1.

ABSTRACT: Na⁺/Ca²⁺ exchangers (NCX) transport Ca²⁺ in or out of cells in exchange for Na⁺. They are ubiquitously expressed and play an essential role in maintaining cytosolic Ca²⁺ homeostasis. Although extensively studied, little is known about the global structural arrangement of eukaryotic NCXs and the structural mechanisms underlying their regulation by various cellular cues including cytosolic Na⁺ and Ca²⁺. Here we present the cryo-EM structures of human cardiac NCX1 in both inactivated and activated states, elucidating key structural elements important for NCX ion exchange function and its modulation by cytosolic Ca²⁺ and Na⁺. We demonstrate that the interactions between the ion-transporting trans

SUBMITTER: Xue J 

PROVIDER: S-EPMC10550945 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature