Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice.

ABSTRACT: Engineering human tissue with diverse cell types and architectures remains challenging. The cerebral cortex, which has a layered cellular architecture composed of layer-specific neurons organised into vertical columns, delivers higher cognition through intricately wired neural circuits. However, current tissue engineering approaches cannot produce such structures. Here, we use a droplet printing technique to fabricate tissues comprising simplified cerebral cortical columns. Human induced pluripotent stem cells are differentiated into upper- and deep-layer neural progenitors, which are then printed to form cerebral cortical tissues with a two-layer organization. The tissues show layer-specific biomarker expression and develop a structurally integrated network of processes. Implantation of t

SUBMITTER: Jin Y 

PROVIDER: S-EPMC10551017 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature