Development of a quantitative thyroid-stimulating hormone assay system for a benchtop digital ELISA desktop analyzer.

ABSTRACT: Regular checkups for thyroid-stimulating hormone (TSH) levels are essential for the diagnosis of thyroid disease. The enzyme-linked immunosorbent assay (ELISA) technique is a standard method for detecting TSH in the serum or plasma of hospitalized patients. A recently developed next-generation ELISA, the digital immunoassay (d-IA), has facilitated detection of molecules with ultra-high-sensitivity. In this study, we developed a TSH assay system using the d-IA platform. By utilizing the ultrasensitivity of d-IA, we were able to use a sample volume of as little as 5 µL for each assay (the dead volume was 5 µL). The limits of blank, detection, and quantification (i.e., functional sensitivity), were 0.000346, 0.001953, and 0.002280 μIU/mL, respectively, and the precision of the total coefficie

SUBMITTER: Arai Y 

PROVIDER: S-EPMC10551129 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature