Functional plasticity in chromosome-microtubule coupling on the evolutionary time scale.

ABSTRACT: The Dam1 complex is essential for mitotic progression across evolutionarily divergent fungi. Upon analyzing amino acid (aa) sequences of Dad2, a Dam1 complex subunit, we identified a conserved 10-aa-long Dad2 signature sequence (DSS). An arginine residue (R126) in the DSS is essential for viability in Saccharomyces cerevisiae that possesses point centromeres. The corresponding arginine residues are functionally important but not essential for viability in Candida albicans and Cryptococcus neoformans; both carry several kilobases long regional centromeres. The purified recombinant Dam1 complex containing either Dad2^ΔDSS or Dad2^R126A failed to bind microtubules (MTs) or form any visible rings like the WT complex. Intriguingly, functional analysis re

SUBMITTER: Sankaranarayanan SR 

PROVIDER: S-EPMC10551642 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature