Diya - A universal light illumination platform for multiwell plate cultures.

ABSTRACT: Recent progress in protein engineering has established optogenetics as one of the leading external non-invasive stimulation strategies, with many optogenetic tools being designed for in vivo operation. Characterization and optimization of these tools require a high-throughput and versatile light delivery system targeting micro-titer culture volumes. Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes. Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough. It offers light induction programming via a user-friendly custom-designed GUI. Through extensive characterization experiments with multiple optogenetic tools in diverse model orga

SUBMITTER: Kumar S 

PROVIDER: S-EPMC10551653 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature