15-keto-PGE₂ alleviates nonalcoholic steatohepatitis through its covalent modification of NF-κB factors.

ABSTRACT: 15-keto-PGE₂ is one of the eicosanoids with anti-inflammatory properties. In this study, we demonstrated that 15-keto-PGE₂ post-translationally modified the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) subunits p105/p50 and p65 at Cys59 and Cys120 sites, respectively, hence inhibiting the activation of NF-κB signaling in macrophages. In mice fed a high-fat and high-sucrose diet (HFHSD), 15-keto-PGE₂ treatment reduced pro-inflammatory cytokines and fasting glucose levels. In mice with non-alcoholic steatohepatitis (NASH) induced by a prolonged HFHSD, 15-keto-PGE₂ treatment significantly decreased liver inflammation, lowered serum levels of alanine transaminase (ALT) and aspartate transferase (AST), and inhibited macrophage in

SUBMITTER: Hee SW 

PROVIDER: S-EPMC10551900 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature