Protein secretion zones during overexpression of amylase within the Gram-positive cell wall.

ABSTRACT:

Background

Whereas the translocation of proteins across the cell membrane has been thoroughly investigated, it is still unclear how proteins cross the cell wall in Gram-positive bacteria, which are widely used for industrial applications. We have studied the secretion of α-amylase AmyE within two different Bacillus strains, B. subtilis and B. licheniformis.

Results

We show that a C-terminal fusion of AmyE with the fluorescent reporter mCherry is secreted via discrete patches showing very low dynamics. These are visible at many places within the cell wall for many minutes. Expression from a high copy number plasmid was required to be able to see these structures we term "secretion zones". Zones corresponded to visualized AmyE activity on the surface of cells, showing that the

SUBMITTER: Strach M 

PROVIDER: S-EPMC10552229 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature