Hsa_circRNA_102051 regulates colorectal cancer proliferation and metastasis by mediating Notch pathway.

ABSTRACT:

Background

The purpose of this study was to investigate the role of hsa_circRNA_102051 in colorectal cancer (CRC) and its effect on the stemness of tumor cells.

Methods

CircRNA microarray was under analysis to screen differentially expressed novel circRNAs in the pathology of CRC. Quantitative real-time PCR was used to detect the relative RNA expression in CRC cells and samples. The effects of hsa_circRNA_102051 on biological functions in CRC cells were accessed both in vitro and in vivo. FISH, RIP and luciferase reporter assay were conducted to confirm the regulatory correlations between hsa_circRNA_102051 and miR-203a, as well as miR-203a and BPTF. Xenograft models were applied to further verify the impacts and fluctuations of hsa_circRNA_102051/miR-203a/BPTF. Moreover, th

SUBMITTER: Chen Z 

PROVIDER: S-EPMC10552285 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature