The Golgi stacking protein GRASP55 is targeted by the natural compound prodigiosin.

ABSTRACT:

Background

The bacterial secondary metabolite prodigiosin has been shown to exert anticancer, antimalarial, antibacterial and immunomodulatory properties. With regard to cancer, it has been reported to affect cancer cells but not non-malignant cells, rendering prodigiosin a promising lead compound for anticancer drug discovery. However, a direct protein target has not yet been experimentally identified.

Methods

We used mass spectrometry-based thermal proteome profiling in order to identify target proteins of prodigiosin. For target validation, we employed a genetic knockout approach and electron microscopy.

Results

We identified the Golgi stacking protein GRASP55 as target protein of prodigiosin. We show that prodigiosin treatment severely affects Golgi morphology and

SUBMITTER: Berning L 

PROVIDER: S-EPMC10552397 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature