The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition.

ABSTRACT:

Background and objective

Imprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition.

Methods

Thirty-nine overweight/obese and 25 normal weight pregnant women were selected from the "Araraquara Cohort Study" according to their pre-pregnancy BMI. Fetal growth and body composition and newborn growth were assessed, respectively, by ultrasound and anthropometry. The methylation of H19DMR in maternal blood, cord blood, maternal decidua and placental villi tissues was evaluated by methylation-sensitive restriction endonuclease qPCR, and H19 and IGF2 expression by relative real-time PCR quantifica

SUBMITTER: Argentato PP 

PROVIDER: S-EPMC10552537 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature