Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.

ABSTRACT: Studies on the transcriptional control of gene expression are crucial to understand changes in organism's physiological or cellular conditions. To obtain reliable data on mRNA amounts and the estimation of gene expression levels, it is crucial to normalize the target gene with one or more internal reference gene(s). However, the use of constitutive genes as reference genes is controversial, as their expression patterns are sometimes more complex than previously thought. In various arthropod vectors, including ticks, several constitutive genes have been identified by studying gene expression in different tissues and life stages. The cattle tick Rhipicephalus microplus is a major vector for several pathogens and is widely distributed in tropical and subtropical regions globally. Tick develop

SUBMITTER: Kim TK 

PROVIDER: S-EPMC10555470 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature