Immune Regulatory Function of Cancer- Associated Fibroblasts in Non-small Cell Lung Cancer.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) are key components of the tumor microenvironment and significantly contribute to immune evasion. We investigated the effects of CAFs on the immune function of CD4+ and CD8+ T cells in non-small cell lung cancer (NSCLC).

Methods

We isolated CAFs and normal fibroblasts (NFs) from tumors and normal lung tissues of NSCLC patients, respectively. CAFs were co-cultured with activated T cells to evaluate their immune regulatory function. We investigated the effect of CAF conditioned medium (CAF-CM) on the cytotoxicity of T cells. CAFs were also co-cultured with activated peripheral blood mononuclear cells and further incubated with cyclooxygenase- 2 (COX2) inhibitors to investigate the potential role of COX2 in immune evasion.

Resul

SUBMITTER: Lee H 

PROVIDER: S-EPMC10555526 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature