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Correlative montage parallel array cryo-tomography for in situ structural cell biology.

ABSTRACT: Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells. To further extend the flexibility of MPACT, tilt series may be processed in their entirety or as individual tiles suitable for sub-tomogram averaging, enabling efficient data processing and analysis.

SUBMITTER: Yang JE

PROVIDER: S-EPMC10555823 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Correlative montage parallel array cryo-tomography for in situ structural cell biology.

Yang Jie E JE Larson Matthew R MR Sibert Bryan S BS Kim Joseph Y JY Parrell Daniel D Sanchez Juan C JC Pappas Victoria V Kumar Anil A Cai Kai K Thompson Keith K Wright Elizabeth R ER

Nature methods 20230918 10

Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells. To further extend the flexibilit ...[more]

PMID: 37723245

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