Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi.

ABSTRACT: Naegleria gruberi is a free-living amoeboflagellate commonly found in freshwater and in soils around the world. It is a non-pathogenic relative of Naegleria fowleri, which is the etiologic agent of Primary Amoebic Meningoencephalitis (PAM). PAM occurs world-wide and it is considered a rare disease, but its fatality rate is high (96%) mainly because of delay in initiation of treatment due to misdiagnosis and lack of a specific treatment. The analysis of gene expression by quantitative real-time PCR in N. gruberi could be a highly efficient means to understand the pathogenicity of N. fowleri and also to find drug targets. Accurate RT-qPCR analysis requires correct normalization of gene expression data using reference genes (RG), whose expression should be constant under different experimenta

SUBMITTER: Martin-Perez T 

PROVIDER: S-EPMC10555999 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature