The proton channel OTOP1 is a sensor for the taste of ammonium chloride.

ABSTRACT: Ammonium (NH₄⁺), a breakdown product of amino acids that can be toxic at high levels, is detected by taste systems of organisms ranging from C. elegans to humans and has been used for decades in vertebrate taste research. Here we report that OTOP1, a proton-selective ion channel expressed in sour (Type III) taste receptor cells (TRCs), functions as sensor for ammonium chloride (NH₄Cl). Extracellular NH₄Cl evoked large dose-dependent inward currents in HEK-293 cells expressing murine OTOP1 (mOTOP1), human OTOP1 and other species variants of OTOP1, that correlated with its ability to alkalinize the cell cytosol. Mutation of a conserved intracellular arginine residue (R292) in the mOTOP1 tm 6-tm 7 linker specifically decreased responses to NH_4<

SUBMITTER: Liang Z 

PROVIDER: S-EPMC10556057 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature