Muscimol inhibits plasma membrane rupture and ninjurin-1 oligomerization during pyroptosis.

ABSTRACT: Pyroptosis is a cell death process that causes inflammation and contributes to numerous diseases. Pyroptosis is mediated by caspase-1 family proteases that cleave the pore-forming protein gasdermin D, causing plasma membrane rupture and release of pathogenic cellular contents. We previously identified muscimol as a small molecule that prevents plasma membrane rupture during pyroptosis via an unidentified mechanism. Here, we show that muscimol has reversible activity to prevent cellular lysis without affecting earlier pyroptotic events. Although muscimol is a well-characterized agonist for neuronal GABA_A receptors, muscimol protection is not altered by GABA_A receptor antagonists or recapitulated by other GABA_A agonists, suggesting that muscimol acts via a no

SUBMITTER: den Hartigh AB 

PROVIDER: S-EPMC10556065 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature