Preparation of ¹⁸O-labelled azaspiracids for accurate quantitation using liquid chromatography-mass spectrometry.

ABSTRACT: Azaspiracids (AZAs) are a group of polyether marine algal toxins known to accumulate in shellfish, posing a risk to human health and the seafood industry. Analysis of AZAs is typically performed using LC-MS, which can suffer from matrix effects that significantly impact the accuracy of measurement results. While the use of isotopic internal standards is an effective approach to correct for these effects, isotopically labelled standards for AZAs are not currently available. In this study, ¹⁸O-labelled AZA1, AZA2, and AZA3 were prepared by reaction with H₂¹⁸O under acidic conditions, and the reaction kinetics and sites of incorporation were studied using LC-HRMS/MS aided by mathematical analysis of their isotope patterns. Analysis of the isotopic incorporatio

SUBMITTER: Wright EJ 

PROVIDER: S-EPMC10556123 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature