Kalkitoxin attenuates calcification of vascular smooth muscle cells via RUNX-2 signaling pathways.

ABSTRACT:

Background

Kalkitoxin (KT) is an active lipopeptide isolated from the cyanobacterium Lyngbya majuscula found in the bed of the coral reef. Although KT suppresses cell division and inflammation, KT's mechanism of action in vascular smooth muscle cells (VSMCs) is unidentified. Therefore, our main aim was to investigate the impact of KT on vascular calcification for the treatment of cardiovascular disease.

Objectives

Using diverse calcification media, we studied the effect of KT on VSMC calcification and the underlying mechanism of this effect.

Methods

VSMC was isolated from the 6 weeks ICR mice. Then VSMCs were treated with different concentrations of KT to check the cell viability. Alizarin red and von Kossa staining were carried out to examine the calcium depos

SUBMITTER: Shrestha SK 

PROVIDER: S-EPMC10556282 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature