Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication.

ABSTRACT:

Background

Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells.

Objectives

PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response.

Methods

Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4⁺ and CD8⁺ T cells after PPRV infection.

Results

PPRV stimulated the differentiation of CD4⁺ and CD8

SUBMITTER: Li L 

PROVIDER: S-EPMC10556287 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature