Effects of biochar amendment and organic fertilizer on microbial communities in the rhizosphere soil of wheat in Yellow River Delta saline-alkaline soil
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ABSTRACT:
SUBMITTER: Li M
PROVIDER: S-EPMC10556502 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
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Project description:Biochar has been widely reported to improve soil conditions and affect plant growth. However, its effectiveness is limited by soil type and production technology. Considering the application effect of biochar in saline alkali soil, there is currently a lack of in-depth mechanism explanations in the research. Therefore, we designed an experiment to explore the effect of biochar on plant growth in saline alkali soil and conducted soil column experiments in a greenhouse environment using composite inorganic fertilizer (NPK). The results showed that biochar significantly affected the distribution of soil nutrient content at different depths, with a significant increase in fertility levels in the surface and middle layers and a decrease in fertility levels in deep soils. Compared to using fertilizers alone, the combined use of biochar and fertilizers further expands the enrichment effect and significantly reduces the leaching of fertilizers into deeper layers. At the same time, the application of biochar also improved soil properties, including an increase in electrical conductivity and organic matter content, as well as an increase in soil enzyme activity. On the other hand, the application of biochar also increases the activity of antioxidant enzymes and the content of osmoregulation substances in plants, reducing the environmental stress that plants are subjected to. Therefore, our results indicate that biochar can reduce the leaching of fertilizers into deep soil layers, improve soil properties, and promotes the growth of Miscanthus in saline alkali soils.
| S-EPMC10609680 | biostudies-literature
Project description:Biochar and bioorganic fertilizers (BOF) that are used in agriculture can, both directly and indirectly, impact rhizosphere soil microorganisms. However, changes to the halophyte rhizosphere bacterial community after applying biochar and BOF to saline−alkali soil have not been thoroughly described. This study has investigated the bacterial communities of halophytes in saline−alkali soil through the addition of different biochar and BOF formulas using Illumina-based sequencing of the 16S rRNA gene fragment. B_BOF (biochar and BOF combined application) had the best effect, either by promoting the plant growth or by improving the physical and chemical properties of the soil. The concentration of the rhizosphere bacterial communities correlated with the changes in soil organic matter (OM) and organic carbon (OC). Proteobacteria, Actinobacteria, Chloroflexi, and Acidobacteria accounted for >80% of the total bacteria in each treatment. In addition, the abundance of Micromonospora was much higher in response to B_BOF than to the other treatments. BOF, with or without biochar, significantly influenced the bacterial community composition in the saline−alkali soil. The OC, OM, total nitrogen, and the available phosphorus had significant effects on the bacterial structure of this soil. The complex correlation of the bacterial communities between CK and B_BOF was higher compared to that between CK and FB or between CK and BOF. These findings suggested that the plant growth, the soil characteristics, and the diversity or community composition of the rhizosphere bacteria in saline−alkali soil were significantly influenced by B_BOF, followed by BOF, and then biochar; fine biochar had a stronger effect than medium or coarse biochar. This study provides an insight into the complex microbial compositions that emerge in response to biochar and BOF.
| S-EPMC9785793 | biostudies-literature
Project description:The utilization of industrial and agricultural resources, such as desulfurization gypsum and straw, is increasingly favored to improve saline alkali land. However, there is still a lack of comprehensive study on the mechanism of organic carbon turnover under the conditions of desulfurization gypsum and straw application. We studied the changes in soil chemical performance, microbial diversity, and microbial community structure in soils with the addition of various levels of straw (no straw, S0; low straw, Sl; medium straw, Sm; and high straw, Sh) and gypsum (no gypsum, DG0; low gypsum, DGl; and high gypsum, DGh) in a 120-day incubation experiment. The bacterial and fungal community richness was higher in the SmDGl treatment than in the SmDG0 treatment. The microbial community evenness showed a similar pattern between the SmDGl and SmDG0 treatments. The combination of the straw and desulfurization gypsum treatments altered the relative abundance of the main bacterial phyla Bacteroidetes and Firmicutes and the dominant fungal class Sordariomycetes, which increased with the enhancement of the SOC ratio. The combination of the straw and desulfurization gypsum treatments, particularly SmDGl, significantly decreased the soil pH and exchangeable sodium percentage (ESP), while it increased the soil organic carbon, microbial biomass carbon, and activities of soil enzymes. Improvement in the soil salinization environment clearly drove the changes in bacterial α-diversity and community, particularly those in the soil carbon fractions and ESP. In conclusion, these findings provide a strong framework to determine the impact of application practices on soil restoration, and the information gained in this study will help to develop more sustainable and effective integrated strategies for the restoration of saline-alkali soil.
| S-EPMC10539599 | biostudies-literature
Project description:Biochar has extensively been used for multiple purposes in agriculture, including improving soil microbial biomass. The current study aimed to investigate the effect of acidic biochar on maize seedlings' rhizosphere bacterial abundance under salinity. There were seven treatments and three replicates in a controlled greenhouse coded as B0S1, B1S1, and B2S1 and B0S2, B1S2, and B2S2. CK is control (free of biochar and salt); B0, B1, and B2 are 0, 15, and 30 g biochar (kg soil)-1; and S1 and S2 are 2.5 and 5 g salt pot-1 that were amended, respectively. After harvesting the maize seedlings, the soil samples were collected and analyzed for soil microbial biomass, bacterial abundance, and diversity. The results revealed that relative abundance of Proteobacteria, Actinobacteria, and Chloroflexi increased on phylum level, whereas Actinomarinales, Alphaproteobacteria, and Streptomyces enhanced on genus level, respectively, in B2S1 and B2S2, when compared with CK and non-biochar amended soil under saline conditions. The relative abundance of Actinomarinales was positively correlated with total potassium (TK) and Gematimonadetes negatively correlated with total phosphorus (TP). Biochar addition slightly altered the Ace1, Chao1, and alpha diversity. Principal component analysis corresponded to the changes in soil bacterial community that were closely associated with biochar when compared with CK and salt-treated soils. In conclusion, acidic biochar showed an improved soil microbial community under salinity.
| S-EPMC8712691 | biostudies-literature
Project description:Biochar as a soil amendment has been regarded as a promising way to improve soil fertility. However, the response of microbial community after biochar and biochar compound fertilizer (BCF) application has not been thoroughly elucidated. This study evaluated the changes in abundance and composition of bacterial and fungal communities using quantitative real-time PCR (qPCR) and Illumina MiSeq amplicon sequencing. The field experiment ran for 3 years and comprised five treatments: chemical fertilizer as control (CK), straw-returning combined with chemical fertilizer (CS), low biochar application combined with chemical fertilizer (LB), high biochar application combined with chemical fertilizer (HB) and BCF. The results showed that biochar amendment results no changes in the abundance and diversity of bacteria in the bulk and rhizosphere soils. However, the abundance of soil fungi was significantly increased by biochar amendment (LB and HB). LB treatment significantly increased the fungal alpha diversity, while there was no significant change under HB. Furthermore, the dominant bacterial phyla found in the samples were Proteobacteria, Actinobacteria, and Acidobacteria. Biochar addition increased the relative abundance of Actinobacteria in both bulk and rhizosphere soils. The dominant fungal phyla were Ascomycota, Mortierellomycota, and Basidiomycota. The relative abundance of Ascomycota significantly decreased, but Mortierellomycota significantly increased in LB and HB. In addition, redundancy analysis indicated that the changes in bacterial and fungal communities are associated with soil properties such as SOC and TN, which are crucial contributors in regulating the community composition. This study is expected to provide significant theoretical and practical knowledge for the application of biochar in agricultural ecosystem.
| S-EPMC8187762 | biostudies-literature
Project description:High soil salinity is the main factor that limits soil microbial activity in the Yellow River Delta (YRD); however, its effects on fungal community and ecological function are unknown. Here, we comparatively investigated the diversity and structures of soil fungal communities targeting the internally transcribed fungal spacer gene using Illumina MiSeq sequencing methods under a salt gradient with five levels, namely, Low: low-salinity soil, Medium: medium-salinity soil, High: high-salinity soil, Extreme: extreme-salinity soil, and a non-salted site as the control (Non-saline). The results show that bulk density (BD) values significantly increased (p < 0.05), while significantly lower values of soil total carbon (TC), total nitrogen (TN), and fungal Shannon and Chao indexes were observed as the salinization gradient increased (p < 0.05). The relatively high levels of the families Nectriaceae and Cladosporiaceae distinguished two of the clusters, indicating two enterotypes of low (Non-saline and Low) and high (Medium, High, and Extreme) salinity soils, respectively. The family Nectriaceae was most abundant in the networks, and the positive correlations were more pronounced than negative correlations; however, Cladosporiaceae was the family most negatively correlated with others based on the network analysis. At the ecological function level, plant saprotrophs and litter saprotroph were significantly less abundant in extremely saline soil than non-saline soil. The change in soil properties (TC, TN, and BD) caused by soil salinization [salt and electrical conductivity (EC)] regulated the diversity of soil fungal communities, and ecological function, as indicated by Pearson correlation analyses. We suggest further investigation into the ecological functions of soil microorganisms in the extremely saline-alkaline soils of the YRD.
| S-EPMC7786015 | biostudies-literature
Project description:Biochar amendment can influence the abundance, activity, and community structure of soil microbes. However, scare information is present about the effect of the combined application of biochar with synthetic nitrogen (N) fertilizer under paddy field condition. We aimed to resolve this research gap in rice field conditions through different biochar in combination with N fertilizers on soil nutrients, soil microbial communities, and rice grain yield. The present study involves eight treatments in the form of biochar (0, 10, 20, and 30 t ha-1) and N (135 and 180 kg ha-1) fertilizer amendments. The soil microbial communities were characterized using high-throughput sequencing of 16S and Internal transcribed spacer (ITS) ribosomal RNA gene amplicons. Experiential findings showed that the treatments had biochar amendments along with N fertilizer significantly advanced soil pH, soil organic carbon (SOC), total nitrogen (TN), soil microbial carbon (SMBC), soil microbial nitrogen (SMBN), and rice grain yield in comparison to sole N application. Furthermore, in comparison with control in the first year (2019), biochar amendment mixed with N fertilizer had more desirable relative abundance of microorganism, phyla Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia with better relative abundance ranging from 8.49, 4.60, 46.30, and 1.51% in T7, respectively. Similarly, during 2020, bacteria phyla Acidobacteria, Actinobacteria, Bacteroidetes, Gemmatimonadetes, Planctomycetes, and Verrucomicrobia were resulted in higher and ranging from 8.69, 5.18, 3.5, 1.9, 4.0, and 1.6%, in biochar applied treatments, respectively, as compared to control (T1). Among the treatments, Sphingopyxis and Thiobacillus bacterial genus were in higher proportion in T7 and T3, respectively, as compared to other treatments and Bacillus was higher in T6. Interestingly, biochar addition significantly decreased the soil fungi phyla Ascomycota, Basidiomycota, Chytridiomycota, and Rozellomycota, in 2020 as compared to 2019. Whereas biochar addition to soil decreased Echria, Kohlmeyeriopsis, and Westerdykella fungal genus as compared to non-biochar treatments. The redundancy analysis showed that soil biochemical traits were positively correlated with soil bacteria. In addition, correlation analysis showed that soil bacteria including Acidobacteria, Actinobacteria, Bacteroidetes, Planctomycetes, and Proteobacteria strongly correlated with rice grain yield. This study demonstrated that soil nutrients and bacteria contribute to an increase in rice yield in combined biochar amendment with lower N treatments.
| S-EPMC8984124 | biostudies-literature
Project description:Saline-alkali soil significantly impairs crop growth. This research employs the impacts of the modifier and water-soluble fertilizer, as well as their interaction, on the root systems of alfalfa and leymus chinensis in saline-alkali soil. The results exhibit that the hydrochar source modifier effectively enhances the root growth of both forage species. There are certain improvements in the root growth indicators of both crops at a dosage of 20 g/kg. Root enzyme activity and rhizosphere soil enzyme activity are enhanced in alfalfa, showing significant improvements in the first planting compared to the second planting. The application of water-soluble fertilizers also promotes root growth and root dehydrogenase activity. The root dehydrogenase activity of alfalfa and leymus chinensis are enhanced 62.18% and 10.15% in first planting than that of blank, respectively. Additionally, the two-factor variance analysis revealed a correlation between rhizosphere soil enzyme activity and changes in root traits. Higher rhizosphere soil enzyme activity is observed in conjunction with better root growth. The combined application of a modifier and water-soluble fertilizer has demonstrated a significant interaction effect on various aspects of the first planting of alfalfa and leymus chinensis. Moreover, the combined application of the modifier and water-soluble fertilizer has yielded superior results when compared to the individual application of either the modifier or the water-soluble fertilizer alone. This combined approach has proven effective in improving saline-alkali soil conditions and promoting crop growth in such challenging environments.
| S-EPMC10903894 | biostudies-literature
Project description:Saline-alkaline conditions can limit crop productivity and the role of soil microbes in nutrient cycling in arid and semi-arid regions throughout the world. A better understanding of how soil amendments and plant varieties affect rhizosphere microbial communities in saline-alkaline environments is important for the development of sustainable and productive agricultural systems under these challenging conditions. The objective of this study was to determine the effect of organic soil amendments on crop yield, soil physicochemical properties and rhizosphere bacterial communities of two oat cultivars in a saline-alkaline soil. The experiment was conducted in a semi-arid region of Northern China and involved growing two oat cultivars with varying levels of saline-alkaline tolerance under four different amendment treatments: (1) control (no amendments), (2) bio-fertilizer, (3) rotten straw, and (4) combination of bio-fertilizer and rotten straw. The combined bio-fertilizer and rotten straw amendment treatment resulted in the highest oat yields, reduced soil pH, and increased soil salt content for both cultivars. Baiyan2 (tolerant cultivar) had a higher bacterial α-diversity, relative abundance of Proteobacteria and Acidobacteria, and lower relative abundance of Firmicutes compared to Caoyou1 (sensitive cultivar). The rotten straw treatment and combined amendment treatment decreased bacterial α-diversity and the abundance of Proteobacteria, and increased the abundance of Firmicutes, which were positively correlated with soil salt, available nitrogen, phosphorous and potassium for both cultivars. Our study suggested using tolerant oat cultivars with the combined application of rotten straw and bio-fertilizer could be an effective strategy in remediating saline-alkaline soils.
| S-EPMC7669890 | biostudies-literature
Project description:The characteristic of particle size distribution (PSD) in the newly formed wetlands in coast has seldom been studied. We applied fractal-scaling theory in assessing soil particle size distribution (PSD) features of newly formed wetlands in the Yellow River Delta (YRD), China. The singular fractal dimensions (D) values ranged from 1.82 to 1.90, the capacity dimension (D0) values ranged from 0.84 to 0.93, and the entropy dimension (D1) values ranged from 0.66 to 0.84. Constrained corresponding analysis revealed that 43.5% of the variance in soil PSD can be explained by environmental factors, including 14.7% by seasonal variation, 8.6% by soil depth, and 8.0% by vegetation type. The fractal dimensions D and D1 were sensitive with fine particles with size ranging less than 126 μm, and D0 was sensitive with coarse particles with size ranging between 126 μm to 2000 μm. Fractal analysis makes full use of soil PSD information, and offers a useful approach to quantify and assess the soil physical attributes in the newly formed wetland.
| S-EPMC4650681 | biostudies-other