Circadian ribosome profiling reveals a role for the Period2 upstream open reading frame in sleep.

ABSTRACT: Many mammalian proteins have circadian cycles of production and degradation, and many of these rhythms are altered posttranscriptionally. We used ribosome profiling to examine posttranscriptional control of circadian rhythms by quantifying RNA translation in the liver over a 24-h period from circadian-entrained mice transferred to constant darkness conditions and by comparing ribosome binding levels to protein levels for 16 circadian proteins. We observed large differences in ribosome binding levels compared to protein levels, and we observed delays between peak ribosome binding and peak protein abundance. We found extensive binding of ribosomes to upstream open reading frames (uORFs) in circadian mRNAs, including the core clock gene Period2 (Per2). An increase in the number of uORF

SUBMITTER: Millius A 

PROVIDER: S-EPMC10556633 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature