Photoactivated Protein Degrader for Optical Control of Synaptic Function.

ABSTRACT: Hundreds of proteins determine the function of synapses, and synapses define the neuronal circuits that subserve myriad brain, cognitive, and behavioral functions. It is thus necessary to precisely manipulate specific proteins at specific sub-cellular locations and times to elucidate the roles of particular proteins and synapses in brain function. We developed PHOtochemically TArgeting Chimeras (PHOTACs) as a strategy to optically degrade specific proteins with high spatial and temporal precision. PHOTACs are small molecules that, upon wavelength-selective illumination, catalyze ubiquitylation and degradation of target proteins through endogenous proteasomes. Here, we describe the design and chemical properties of a PHOTAC that targets Ca²⁺/calmodulin-dependent protein kinase II

SUBMITTER: Ko T 

PROVIDER: S-EPMC10557063 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature