Automated Sample Preparation Workflow for Tandem Mass Tag-Based Proteomics.

ABSTRACT: Although tandem mass tag (TMT)-based isobaric labeling has become a powerful approach for multiplexed protein quantitation, automating the workflow for this technique has not been easy to achieve for widespread adoption. This is because preparation of TMT-labeled peptide samples involves multiple steps ranging from protein extraction, denaturation, reduction, and alkylation to tryptic digestion, desalting, labeling, and cleanup, all of which require a high level of proficiency. The variability resulting from multiple processing steps is inherently problematic, especially with large-scale clinical studies that involve hundreds of samples where reproducibility is critical for quantitation. Here, we sought to compare the performance of a recently introduced platform, AccelerOme, for an automa

SUBMITTER: Mun DG 

PROVIDER: S-EPMC10557128 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature