Good Vibrations Report on the DNA Quadruplex Binding of an Excited State Amplified Ruthenium Polypyridyl IR Probe.

ABSTRACT: The nitrile containing Ru(II)polypyridyl complex [Ru(phen)₂(11,12-dCN-dppz)]²⁺ (1) is shown to act as a sensitive infrared probe of G-quadruplex (G4) structures. UV-visible absorption spectroscopy reveals enantiomer sensitive binding for the hybrid htel(K) and antiparallel htel(Na) G4s formed by the human telomer sequence d[AG₃(TTAG₃)₃]. Time-resolved infrared (TRIR) of 1 upon 400 nm excitation indicates dominant interactions with the guanine bases in the case of Λ-1/htel(K), Δ-1/htel(K), and Λ-1/htel(Na) binding, whereas Δ-1/htel(Na) binding is associated with interactions with thymine and adenine bases in the loop. The intense nitrile transient at 2232 cm^-1

SUBMITTER: Stitch M 

PROVIDER: S-EPMC10557146 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature