Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.

ABSTRACT:

Background

Neurosphere medium (NSM) and self-renewal medium (SRM) were widely used to isolate enteric neural stem cells (ENSCs) in the form of neurospheres. ENSCs or their neurosphere forms were neurogenic and gliogenic, but the compelling evidence for their capacity of assembling enteric neural networks remained lacking, raising the question of their aptitude for rebuilding the enteric nervous system (ENS) in ENSC therapeutics. It prompted us to explore an effective culture protocol or strategy for assembling ENS networks, which might also be employed as an in vitro model to simplify the biological complexity of ENS embedded in gut walls.

Methods

NSM and SRM were examined for their capacity to generate neurospheres in mass culture of dispersed murine fetal enterocytes at se

SUBMITTER: Chen JC 

PROVIDER: S-EPMC10557225 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature